5-Deazaflavin (≥98% HPLC) | Next-Generation Longevity Coenzyme for Mitochondrial ATP Amplification & Sirtuin Activation
1. Product Overview & Value Proposition
5-Deazaflavin (5-Deazaisoalloxazine, CAS: 26908-38-3) is a synthetic deaza-analogue of riboflavin (Vitamin B2) and a core functional mimetic of coenzyme F420—a redox cofactor naturally specialized in obligate two-electron (hydride) hydride transfers. In 5-deazaflavin, the nitrogen atom at position 5 of the isoalloxazine ring is substituted by a carbon-hydrogen group (=CH-), transforming the typical flavin single-electron radical mechanism into a direct two-electron hydride-transfer coenzyme. This molecular configuration yields an extraordinarily negative standard redox potential (approximately -650 mV), vastly outperforming the energetic driving force of traditional NAD+/NADH couples (-320 mV). In emerging geroscience and advanced longevity medicine, 5-Deazaflavin powder has earned international acclaim as a “next-generation cellular energy engine.” It operates independently of the rate-limiting NAMPT enzyme and CD38 degradation pathways, directly stimulating mitochondrial ATP synthesis and potently activating Sirtuin longevity proteins (SIRT1 and SIRT3). Formulators worldwide deploy this cutting-edge advanced longevity raw material to develop high-performance cellular rejuvenation capsules, executive stamina supplements, and mitochondrial resilience matrices.
As an established bulk 5-Deazaflavin manufacturer and direct longevity ingredient supplier, we supply certified pharmaceutical- and dietary supplement-grade crystalline powders standardized to not less than 98.0% active purity (with premium lots exceeding 99.0% by HPLC). Produced via multi-step stereospecific catalytic synthesis and multi-stage low-temperature recrystallization, our material is free from unreacted intermediates, heavy metals, and toxic chemical solvents. To empower pioneering nutrition brands and clinical researchers, we combine rigorous analytical standards with client-centered commercial flexibility: accessible low MOQ procurement for benchtop pilot trials, multi-ingredient shipment consolidation across our longevity active, nucleotide, and antioxidant catalog, and dedicated on-the-ground China-wide raw material sourcing assistance tailored to your exact manufacturing requirements.
2. Product Specifications (Analytical Quality)
| Test Item | Specification Standard (Oral / Food Grade) | Test Method |
|---|---|---|
| Identification (IR / NMR / HPLC) | Conforms to 5-Deazaflavin Reference Standard | FTIR / 1H-NMR / Retention Time Matching |
| Assay (5-Deazaflavin on Dry Basis) | ≥ 98.0% – 101.0% (Typically ≥ 99.0%) | HPLC |
| Related Chemical Impurities | Individual Impurity ≤ 0.5%; Total Impurities ≤ 1.5% | HPLC |
| Appearance | Light yellow to yellow-orange crystalline fine powder | Visual / Organoleptic |
| Melting Range | 310.0°C – 315.0°C (Decomposition) | Capillary Melting Point Apparatus |
| Particle Size | 100% passing 80 mesh (Micronized d90 ≤ 15 μm available) | Sieve Analysis / Laser Diffraction |
| Loss on Drying | ≤ 1.0% | Gravimetric (105°C) |
| Residue on Ignition (Ash) | ≤ 0.20% | Gravimetric (600°C) |
| Heavy Metals (Pb) | < 0.5 ppm (As < 0.5 ppm, Cd < 0.1 ppm, Hg < 0.1 ppm) | ICP-MS |
| Residual Solvents (Ethanol, DMF, EtOAc) | Meets USP <467> / EP Option 1 guidelines | GC-HS |
| Microbiological Limits | Total Plate Count ≤ 1,000 CFU/g; Yeast & Mold ≤ 100 CFU/g | USP/EP Standard |
3. Functional Applications (Mechanism of Action)
The targeted biological applications of 5-Deazaflavin across cellular bioenergetics, epigenetic regulation, and systemic longevity are driven by distinct biophysical and enzymatic mechanisms:
- Direct Electron Transport & Mitochondrial ATP Amplification: Due to its ultra-low standard reduction potential (~ -650 mV), 5-deazaflavin serves as a potent electron donor to mitochondrial respiratory chains. It fuels electron flux and proton pumping across the inner mitochondrial membrane, boosting ATP generation without imposing cellular metabolic bottlenecks.
- Direct, Potent Sirtuin (SIRT1 & SIRT3) Activation: Demonstrates remarkable potency in upregulating Sirtuin deacetylases (SIRT1 in nuclei, SIRT3 in mitochondria), promoting mitochondrial biogenesis (via PGC-1α deacetylation), improving oxidative resilience, and stabilizing chromatin architecture.
- Independence from CD38 Degradation & NAMPT Pathways: Unlike standard NAD+ precursors (NMN and NR) that require functional salvage pathway enzymes (NAMPT) and suffer rapid enzymatic breakdown by age-upregulated CD38 ectoenzymes, 5-deazaflavin is resistant to CD38-mediated cleavage, providing sustained cellular efficacy.
- Two-Electron (Hydride) Transfer Without Radical Generation: The =CH- group at position 5 restricts the molecule to clean, obligate two-electron transfers, bypassing single-electron semiquinone radical intermediates. This prevents the generation of reactive oxygen species (ROS) during cellular redox cycling.
- Telomere Maintenance & DNA Integrity Support: Preclinical models demonstrate that 5-deazaflavin supplementation supports telomerase reverse transcriptase (TERT) activity and enhances PARP-mediated DNA repair signaling, counteracting chromosomal degradation during cellular replication.
4. Formulation Combinations
Maximize your finished longevity supplement impact by pairing our high-purity 5-Deazaflavin with complementary mitochondrial, cellular energy, and sirtuin actives:
- The Ultimate Dual-Engine Longevity Powerhouse: Our 5-Deazaflavin (30–50 mg) + Beta-Nicotinamide Mononucleotide (β-NMN ≥99%, 300–500 mg) + PQQ Disodium Salt (≥98%, 10–20 mg). A revolutionary multi-tier energy matrix: β-NMN replenishes endogenous NAD+ coenzyme pools, PQQ drives the biogenesis of new mitochondrial organelles, and 5-Deazaflavin injects high-potential electron transfer to maximize ATP output and Sirtuin deacetylation.
- The Epigenetic Clock & Sirtuin Synergist Complex: Our 5-Deazaflavin + Pterostilbene 99% + Calcium Alpha-Ketoglutarate (Ca-AKG ≥98%) + Apigenin 98%. An advanced formulation engineered to combine high-energy coenzyme mimicry with epigenetic DNA demethylation, allosteric SIRT1 activation, and natural CD38 inhibition.
5. Why Choose Us As Your Long-Term Longevity Partner?
- Flexible Low MOQ Policy: Eliminate inventory barriers. As an agile low MOQ 5-deazaflavin distributor, our orders start as low as 100 g to 1 kg for bench-scale pilot trials and formulation testing, scaling smoothly to recurring commercial manufacturing contracts.
- Multi-Product Portfolio Consolidation: Streamline your supply chain by sourcing your next-generation coenzymes (5-Deazaflavin), direct NAD+ boosters (β-NMN, NR-Cl), autophagy activators (Spermidine 3HCl), senolytics (Fisetin), and epigenetic regulators (Ca-AKG) under a single, cost-effective freight shipment to reduce landed customs costs.
- Value-Added China Sourcing Service: In addition to our dedicated chemical and bio-synthesis manufacturing lines, our on-the-ground technical team audits facilities, validates chromatographic testing paperwork, and procures specialized functional ingredients across China tailored to your exact manufacturing criteria.
6. Quality & Supply Chain Assurance
- High-Precision Catalytic Synthesis: Synthesized through controlled heterocyclic condensation using pharmaceutical-grade reagents, avoiding the low-yield and non-selective by-products of rudimentary synthetic routes.
- Validated Spectral Fingerprinting (NMR / MS / HPLC): Every batch is analytically verified via high-resolution Liquid Chromatography-Mass Spectrometry (LC-MS) and Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy to confirm exact chemical identity, molecular weight, and absence of deaza-isomer structural impurities.
- High Flowability & Stability: 5-Deazaflavin possesses a high melting point (>310°C) and superior thermal stability compared to heat-sensitive nucleotides, ensuring excellent shelf-life and smooth operation on high-speed automated capsule filling equipment.
- Hermetic Moisture- & Light-Proof Packaging: Vacuum-sealed in multi-layer aluminum foil barrier pouches (100 g, 500 g, 1 kg) inside durable 25 kg fiber export drums with pharmaceutical-grade desiccants to shield this light-sensitive crystalline powder from ambient humidity and photo-oxidation during global transit.
7. FAQ
Q1: How does 5-Deazaflavin compare to traditional NAD+ boosters like NMN and NR?
A1: While NMN and NR are biological precursors that rely on multi-step intracellular enzymatic cascades (such as NMNAT and NRK) to synthesize NAD+, 5-Deazaflavin functions as a direct redox coenzyme analogue. Possessing an ultra-low redox potential (-650 mV), 5-Deazaflavin transfers electrons with significantly higher energetic efficiency than NAD+ (-320 mV), is not hydrolyzed by the NAD-consuming CD38 enzyme, and activates Sirtuin longevity pathways with exceptionally high potency.
Q2: What is the typical recommended dosage for 5-Deazaflavin in finished formulations?
A2: Because of its concentrated redox potency and direct catalytic activity, 5-Deazaflavin is typically formulated at lower milligram dosages than NMN. Standard finished supplement formulations range from 10 mg to 50 mg per daily serving, often combined with complementary cellular coenzymes or polyphenols for enhanced multi-system synergy.
Q3: What is the MOQ for bulk 5-Deazaflavin powder orders?
A3: We support agile product development with minimum order quantities starting from just 100 g to 1 kg for formulation R&D and analytical validation, scaling seamlessly up to multi-hundred-kilogram commercial production contracts.
8. References
- PubChem: 5-Deazaflavin Compound Summary (CID 100742) – National Center for Biotechnology Information.
- PubMed Central: 5-Deazaflavin and Coenzyme F420 Analogues: Hydride Transfer Mechanisms, Redox Bioenergetics, and Longevity Potential – National Institutes of Health research database.
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